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TNFRSF1A (CD120a) Recombinant Human Protein, His Tag

Catalog number: 10872H08H250

 Related applications: Protein Assays and Analysis

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Description

Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A/CD120a), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Thr 211) of human TNFRSF1A (NP_001056.1) fused to a polyhistidine tag at the C-terminus.

Activity:
1. Measured by its binding ability in a functional ELISA:
a. Immobilized human TNFa at 10 µg/ml(100 µl/well) can bind biotinylated human TNFRSF1A-his. The ED50 of biotinylated human TNFRSF1A-his is 0.04 µg/ml.
b. Immobilized mouse TNFa at 10 µg/ml(100 µl/well) can bind biotinylated human TNFRSF1A-his. The ED50 of biotinylated human TNFRSF1A-his is 0.28 µg/ml.
2. Measured by its ability to inhibit the TNF mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.04-0.2 µg/mlin the presence of 0.25 ng/mlrecombinant human TNF.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: CD120a, FPF, MGC19588, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR60, p55, p55-R, p60
Gene ID: 7132
Gene Symbol: TNFRSF1A
Molecular Weight: 22.7 kDa
Product Size: 5 x 50 µg
Protein Family: CD Proteins, Cytokines & Receptors
Protein Form: Recombinant, Truncated
Protein Subtype: TNF (Tumor Necrosis Factor)
Protein Tag or Fusion: His Tag
Purity: ≥ 90% by SDS-PAGE
Research Category: Apoptosis, Dermatology, Genetic Diseases, Immunology, Inflammation, Signal Transduction
Species: Human
Validated Application: Assay Standard
Shipping Condition: Room Temperature

Contents & storage

Store at -20 to -70 °C

Documents

Manuals & protocols

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