Dynabeads® CD15

Catalog number: 11137D

Invitrogen™  Related applications: Cell Isolation & Expansion

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Description

Dynabeads® CD15 are superparamagnetic beads covalently coupled with an anti-human CD15 antibody that efficiently depletes human CD15+ myeloid cells (predominantly neutrophil and eosinophil granulocytes) directly from whole blood, buffy coat, or PBMC samples. Dynabeads® CD15 can also be used to positively isolate CD15+ cells for molecular applications. Advantages of Dynabeads® CD15:

• Efficient depletion of granulocytes (neutrophils and eosinophils)
• Isolation of granulocytes directly from whole blood for molecular applications
• No columns required

About Dynabeads® CD15
Dynabeads® CD15 are uniform, superparamagnetic polystyrene beads (4.5 µm diameter) coated with a monoclonal mouse IgM antibody recognizing the human CD15 membrane antigen. The CD15 antigen is predominantly expressed on human neutrophil and eosinophil granulocytes, and to a varying degree on monocytes.

Magnetic bead-;based separation offers easy handling
Dynabeads® CD15 is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, PBMC, or buffy coat.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Capacity: Processes ~2x10^9 cells total
Cell Type: Granulocytes
Product Size: 5 mL
Target Species: Human
Isolation Method: Depletion, positive isolation for molecular applications
Output Viability: >95%
Sample Type (Specific): Blood, PBMC
Starting Material (Cell #): 1x10^7 PBMCs per isolation
Shipping Condition: Room Temperature

Contents & storage

This Product Contains: 5 ml Dynabeads® CD15 coated with anti-CD15 monoclonal antibody.

Storage Conditions: 2°C to 8°C. Do not freeze.

Documents

Manuals & protocols

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