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S100A9 Recombinant Human Protein, His Tag

Catalog number: 11145H08B25

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Manufactured by Sino Biological (China); Distributed by Life Technologies.

S100 Calcium Binding Protein A9 (S100A9) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in performing cell-based assays and protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein is expressed from a DNA sequence encoding the human S100A9 (NP_002956.1) (Met 1-Pro 114) fused to a polyhistidine tag at the C-terminus.

N-terminal Sequence Analysis: : Met.

1. Measured by its ability to bind recombinant human S100A8-his in a functional ELISA.
2. Measured by its ability to bind recombinant human his-S100A8 in a functional ELISA.
3. Measured by its ability to inhibit the proliferation of MCF7 human breast adenocarcinoma cells. The ED50 for this effect is typically 10-30 µg/mL.

Formulation: lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% mannitol, 5% trehalose, 0.02% Tween®-80.

Reconstitution: Dissolve the protein in sterile double-distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: expires one year from date of receipt when stored as instructed.

Human Protein S100-A9, also known as S100 calcium-binding protein A9, is a calcium-binding protein. It has anti-microbial activity towards bacteria and fungi. The anti-microbial and proapoptotic activity of S100A9 is inhibited by zinc ions. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS). It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. S100A9 plays a role as a proinflammatory mediator in acute and chronic inflammation and upregulates the release of IL8 and cell-surface expression of ICAM1.

You may also be interested in the following S100A9 products:
Enzyme & Protein Assays
Gene Expression Assays
For Research Use Only. Not for use in diagnostic procedures.


Form: Lyophilized
Purity: ≥ 90% by SDS-PAGE
Species: Human
Expression: Insect
Gene Symbol: S100A9
Gene Aliases: 60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14,NIF, P14
Product Size: 5 x 5 µg
Protein Family: Other Proteins
Endotoxin Level: < 1 EU/µg
Protein Subtype: Other Proteins
Molecular Weight: 14.6 kDa
Research Category: Inflammation, Microbiology, Cell Cycle & Proliferation
Protein Tag or Fusion: His Tag
Validated Application: Assay Standard

Contents & storage

Includes 5 vial of lyophilized protein. Store at -20 °C.


Manuals & protocols