Dynabeads® CD2

Catalog number: 11159D

Invitrogen™  Related applications: Cell Isolation & Expansion

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Description

Dynabeads® CD2 are magnetic beads that provide a robust and versatile tool to isolate or deplete human CD2+ cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests.
• Rapid isolation of CD2+ cells from any sample – no columns required
• Positive isolation for molecular downstream assays or efficient depletion of CD2+ cells.

Excellent Yield, Purity, and Viability of Recovered Cells
Dynabeads® CD2 are uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of human CD2+ cells directly from any sample. Dynabeads® CD2 beads are coated with a primary monoclonal antibody specific for the CD2 membrane antigen mainly expressed on human T-cells and natural killer (NK) cells. Dynabeads® CD2 bind to CD2+ cells during a short incubation period in a tube, after which the bead-bound cells are separated from unbound cells using a magnet. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the isolated CD2+ cells.

Ideal for Both Depletion and Positive Isolation for Downstream Molecular Assays
Because of the powerful magnets used for separation, Dynabeads® CD2 can be used in viscous samples such as whole blood and bone marrow, thereby providing a convenient method for the efficient depletion of CD2+ cells in about 30 minutes. Dynabeads® CD2 will also afford excellent recovery of high-purity, viable cells when used for positive isolation for downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate T-cells for cell-based applications, or you need to check your samples with flow cytometry, use Dynabeads® FlowComp™ Human CD3 (for bead-free cells) or Dynabeads® Untouched™ Human T Cells to obtain untouched human T cells.

Select what is right for you from our range of Human T Cell Isolation Products.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Capacity: Processes ~2x10^9 cells total
Cell Type: T Cells (Whole Population)
Product Size: 5 mL
Target Species: Human
Isolation Method: Depletion, positive isolation for molecular applications
Output Viability: >95%
Sample Type (Specific): Blood, PBMC
Starting Material (Cell #): 1x10^7 PBMCs per isolation
Shipping Condition: Room Temperature

Contents & storage

This Product Contains: 5 ml Dynabeads® CD2 Pan T coated with anti-CD2 monoclonal antibody.
Store at 2-8°C

Documents

Manuals & protocols

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