Grace's Insect Medium, unsupplemented, powder

Catalog number: 11300027

Gibco™  Related applications: Insect Cell Culture

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Description

Grace's Insect Medium was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. One supplemented, Grace's Unsupplemented Insect Medium is now widely used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21. Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS). Gibco® Grace's Unsupplemented Insect Medium can be used during transfection of insect cells, or for protein labeling studies (with methionine). We offer a variety of Gibco® Media options for a range of insect cell culture applications.

This Grace's Insect Medium is modified as follows:

WithWithout
• L-glutamine • Lactalbumin hydrolysate
• Yeastolate
• Sodium bicarbonate

The complete formulation is available. Grace's Unsupplemented Insect Medium does not contain lactalbumin hydrolysate and yeastolate commonly found in the supplemented version.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco® Grace's Unsupplemented Insect Medium is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Grace's Unsupplemented Insect Medium requires supplementation with 10% Fetal Bovine Serum (FBS), 3.33 g/L lactalbumin hydrolysate and 3.33 g/L yeastolate before use as a growth medium. Grace's Unsupplemented Insect Medium requires ambient CO2 in order to maintain physiological pH. Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment and filtration at the time of preparation (see protocol for details).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Powder
Cell Type: Insect Cells
Glutamine: L-Glutamine
HEPES Buffer: No HEPES
Product Size: 10 x 1 L
Phenol Red Indicator: No Phenol Red
Sodium Pyruvate Additive: No Sodium Pyruvate
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture

Documents

Manuals & protocols

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