Platinum® PCR SuperMix

Catalog number: 11306016

Invitrogen™  Related applications: PCR

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Description

Platinum® PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers, reducing set-up time by half (see figure). Platinum® PCR SuperMix:

• Allows for room temperature reaction assembly
• Amplifies fragments up to 5 kb
• Produces products with 3'-A ends
• Improves PCR specificity

Using Platinum® PCR SuperMix
Platinum® PCR SuperMix is for high-specificity DNA amplification. High specificity is provided by a mixture of recombinant Taq DNA Polymerase and Platinum® monoclonal antibodies to Taq DNA Polymerase. Automatic "hot-start" improves the specificity of PCR. Taq DNA Polymerase is activated during the initial denaturation step.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: Platinum® Taq DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Reaction Format: SuperMix or Master Mix
Product Overhang: 3'-A
Reaction Speed: Standard
Final Product Size(s): 5 kb or less
Product Size: 100 reactions
Exonuclease Activity: 5' - 3'
Shipping Condition: Dry Ice

Contents & storage

Platinum® PCR SuperMix is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 5,000 amplification reactions of 50 µl each are provided.

Platinum® PCR SuperMix Components:
22 U/ml complexed recombinant Taq DNA polymerase with Platinum® Taq Antibody, 22 mM Tris-HCl (pH 8.4), 55 mM KCl, 1.65 mM MgCl2, 220 µM dGTP, 220 µM dATP, 220 µM dTTP, 220 µM dCTP, and stabilizers.

100 rxns: 4 x 1.125 ml

Store at -20°C in a non-frost-free freezer.

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