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Platinum® PCR SuperMix

Catalog number: 11306081

Invitrogen™  Related applications: PCR

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Description

Invitrogen™ Platinum™ PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. All you have to do is add template and primers. Platinum PCR SuperMix:

• Allows for room temperature reaction assembly
• Amplifies fragments up to 5 kb
• Produces products with 3'-A ends
• Improves PCR specificity

Using Platinum PCR SuperMix
Platinum PCR SuperMix is for high-specificity DNA amplification. High specificity is provided by a mixture of recombinant Taq DNA Polymerase and Platinum monoclonal antibodies to Taq DNA Polymerase. Automatic "hot-start" improves the specificity of PCR. Taq DNA Polymerase is activated during the initial denaturation step.

Platinum PCR SuperMix is supplied at 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions. For variable sample volumes exceeding 10% of the final reaction, we recommend using convenient Platinum™ Hot Start PCR 2X Master Mix. Learn more about Platinum Taq DNA Polymerase

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Product Overhang: 3'-A
Final Product Size(s): 5 kb or less
Exonuclease Activity: 5' - 3'
Product Size: 5,000 reactions
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

Platinum PCR SuperMix Components:
22 U/mL recombinant Taq DNA polymerase complexed with Platinum Taq Antibody, 22 mM Tris-HCl (pH 8.4), 55 mM KCl, 1.65 mM MgCl2, 220 µM dGTP, 220 µM dATP, 220 µM dTTP, 220 µM dCTP, and stabilizers.

5,000 rxns: 4 x 56.25 mL

Store at -20°C in a non-frost-free freezer.

Documents

Manuals & protocols