Dynabeads® Human DC Enrichment Kit

Catalog number: 11308D

Invitrogen™  Related applications: Cell Isolation & Expansion

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Description

Use this kit to enrich viable human dendritic cells (DC's) from PBMC by negative isolation. The kit depletes T cells, B cells, monocytes/ macrophages, granulocytes, NK cells and erythrocytes. Highly enriched DC's are bead and antibody-free, and can be used in any functional assay. The cells should be further sorted to obtain high purity of the specific DC sub-populations of choice.

• Obtain high recovery (80-100%) of viable DC's of all sup-populations.
• Substantial reduction in sorting time and volume compared to sorting only, thus saving money as well.

Methodology:
An antibody mix towards the non- dendritic cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining untouched human DC's can be directly sorted into the sup-population of choice or used in any other application.

Starting samples:
PBMC
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Capacity: Processes ~2x10^9 cells total
Cell Type: Dendritic Cells
Product Size: 2 x 10 mL
Target Species: Human
Isolation Method: Negative Isolation
Output Viability: >95%
Sample Type (Specific): PBMC
Starting Material (Cell #): 1x10^7 PBMCs per isolation
Shipping Condition: Room Temperature

Contents & storage

The Kit Contains: 2x10 ml Depletion MyOne™ Streptavidin Dynabeads® and 4 ml Antibody Mix containing an optimized mixture of biotinylated monoclonal antibodies towards CD3, CD14, CD16, CD19, CD56 and Glycophorin A.
Store at 2-8°C

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