Dynabeads® Untouched™ Human NK Cells Kit

Catalog number: 11349D

Invitrogen™  Related applications: Cell Isolation & Expansion

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Description

Use this kit to isolate pure and viable untouched NK cells from PBMC by negative isolation. The kit depletes T cells, B cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human NK cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free NK cells are truly untouched, and in perfect shape for any functional assay and application.

• High purity, recovery and viability of human untouched NK cells
• Easy to use and to scale up
• No columns required

Methodology:
An antibody mix towards the non-NK cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human NK cells can be directly analyzed in a flow cytometer and used in any application.

Starting Sample:
PBMC
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Bead
Capacity: Processes ~1x10^9 cells⁄kit
Cell Type: NK Cells
Product Size: 1 kit
Final Product: Cells
Target Species: Human
Isolation Method: Negative Isolation
Output Viability: >99%
Number of Reactions: 20 Reactions
Sample Type (Specific): MNC
Starting Material (Cell #): ~5x10^7 cells/test
Shipping Condition: Room Temperature

Contents & storage

The Kit Contains: 10 ml Depletion MyOne™ SA Dynabeads® and 2 ml Antibody Mix with biotinylated monoclonal antibodies towards human CD3, CD14, CD36, HLA Class II, CD123 and CD235a (Glycophorin A).
Store at 2-8°C

Documents

Manuals & protocols