Dynabeads® Regulatory CD4+/CD25+ T Cell Kit

Catalog number: 11363D

Invitrogen™  Related applications: Cell Isolation & Expansion

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Description

Use this kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express the intracellular transcription factor Foxp3. The CD4+ CD25- cell fraction can be used as effector cells in downstream inhibitory assays. You can expect >95% purity (CD4+ CD25+ expression) and >80% Foxp3 expression.

Isolate bead- and antibody-free human CD4+ CD25+ Foxp3+ Treg cells

Treg cells can be expanded with Dynabeads® CD3/CD28 Treg Expander and remain
Foxp3+.

Treg cells can be used in any downstream applications (e.g., suppression assays)

Methodology

The isolation procedure includes the following steps:

• Negative isolation of untouched human CD4+ T-cells from PBMC.
• Positive isolation of CD25+ T-cells from the untouched CD4+ T-cell subset.
• Removal of beads from the CD4+CD25+ Treg cells with DETACHaBEAD®.

Starting sample: PBMC
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Capacity: Processes ~1x10^9 cells total
Cell Type: T Cells (Regulatory)
Product Size: 2 x 5 mL
Target Species: Human
Isolation Method: Depletion, then positive isolation
Output Viability: >95%
Sample Type (Specific): PBMC
Starting Material (Cell #): 1x10^8 PBMCs per isolation
Shipping Condition: Room Temperature

Contents & storage

Each kit contains 2 ml Antibody Mix Human CD4, 10 ml Depletion MyOne™ Dynabeads®, 5 ml Dynabeads® CD25 and 2 ml DETACHaBEAD®. These reagents are sufficient to process ~1×109 cells. Store components at 2-8°C.

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Manuals & protocols

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