Dynabeads® FlowComp™ Human CD3 Kit

Catalog number: 11365D

Invitrogen™  Related applications: Cell Isolation & Expansion

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Description

Dynabeads® FlowComp™ Human CD3 contain magnetic beads that provide a robust and versatile tool to positively isolate pure CD3+ T-cells from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), or tissue digests. The cells can be released from the beads using the FlowComp™ Release Buffer.
• Rapid isolation of CD3+ T-cells from any sample – no columns required
• Pure and bead-free CD3+ T-cells for any downstream application

Excellent Yield, Purity, and Viability of Recovered Cells
Dynabeads® FlowComp™ Human CD3 contains uniform, superparamagnetic beads (2.8 µm diameter) that enable easy isolation of human CD3+ cells directly from any sample via a three-step process. First, the FlowComp™ Human CD3 Antibody is mixed with the sample in a tube where it binds to the target cells during a short incubation. Then, the CD3+ T-cells that have bound the specific antibodies are captured by the FlowComp™ Dynabeads® and separated from unbound cells using a magnet. Finally, the target cells are released from the beads by adding the FlowComp™ Release buffer. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the isolated CD3+ cells. The cells can be used in any downstream application.

Select what is right for you from our range of Human T Cell Isolation Products.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
For Research Use Only. Not for use in diagnostic procedures.

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Specifications

Capacity: 80 ml whole blood or 2x10^9 PBMC
Cell Type: T Cells (CD3)
Final Product: Cells
Form: Bead
Isolation Method: Positive Isolation
Product Size: 1 kit
Purity: 99 %
Sample Type (Specific): Blood, MNC
Target Species: Human
Shipping Condition: Room Temperature

Contents & storage

The Kit contains 1 ml Antibody Mix, 3 ml FlowComp™ Dynabeads® and 40 ml FlowComp™ Release Buffer.
Store at: 2-8°C

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Manuals & protocols

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