Platinum® GenoType Tsp DNA Polymerase

Catalog number: 11448024

Invitrogen™  Related applications: PCR

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Description

Platinum® GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications. The enzyme is pre-complexed with a thermolabile inhibitor containing monoclonal antibodies to Tsp DNA polymerase, providing an automatic "hot-start" method that improves PCR specificity and allows for room temperature reaction assembly. Platinum® GenoType Tsp DNA Polymerase:

• Exhibits minimal non-templated nucleotide addition to PCR products
• Lacks both 5´ and 3´ exonuclease activity
• Amplifies fragments up to 500 bp

Application
Amplification of dinucleotide repeat loci in applications in which elimination of non-templated nucleotide addition is desirable.

Unit definition
One unit of Platinum® GenoType Tsp DNA Polymerase has been functionally determined to be equivalent to one unit of Taq DNA Polymerase in amplification of dinucleotide repeats using standard Taq DNA polymerase reaction conditions. One Tsp DNA polymerase unit approximates 2.5 activity units. An activity unit incorporates 10 nmoles of deoxyribonucleotide into acid-precipitable material in 30 min at 74°C under optimized reaction conditions.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: Tsp Genotyping Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Reaction Format: Separate Components
Product Overhang: Blunt
Reaction Speed: Standard
Final Product Size(s): 500 bp or less
Product Size: 250 units
Exonuclease Activity: None
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

Platinum® GenoType Ts p DNA Polymerase is supplied with 10X PCR Buffer [200 mM Tris-HCl (pH 8.4), 500 mM KCl], and a vial of 50 mM MgCl 2 . Store at -20°C. Guaranteed stable for 6 months when properly stored.

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