This gentle and easy-to-use kit allows you to isolate flow-compatible mouse CD4+ T cells. Immediately after positive isolation, the beads are released and removed from your cell sample. In this way, you avoid exposing your cells to potentially cytotoxic or immunogenic foreign substances like iron oxides or dextrans, a potential problem when working with biodegradable particles. In addition, your cells will not be exposed to the stress of being passed through a column.

You can expect a purity level of >85% and viability of >85% of mouse CD4+ cells.

Advantages:

• No risk of your results being affected by the isolation method
• Gentle tube-based method - no columns needed
• High purity, recovery and viability
• Bead-free cells - compatible with flow cytometry analysis
• Viable, bead-free CD4+ T-cells for direct use in any cell-based assay.

The isolated cells have the presumed profile of memory and naive CD4+ T cells, and show a high proliferation capacity. You will save time and money, and get increased confidence in your results.

Starting sample: Spleen or lymph node cells