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IL21R Recombinant Human Protein, His Tag

Catalog number: 11483H08H250

 Related applications: Protein Assays and Analysis

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Description

Interleukin 21 receptor (IL21R), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the human IL21R (Q9HBE5-1) extracellular domain (Met 1-Pro 236) was expressed, with a polyhistidine tag at the C-terminus.

Activity:
1. Measured by its ability to bind human IL21 in a functional ELISA.
2. Measured by its ability to inhibit IL21-induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 1-5 ng/mlin the presence of 100 ng/mlof recombinant human IL21.
3. Measured by its ability to inhibit IL21 induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 50-200 pg/mlin the presence of 10 ng/mlof recombinant human IL21.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Lyophilized
Purity: ≥ 90% by SDS-PAGE
Gene ID: 50615
Species: Human
Expression: Mammalian
Gene Symbol: IL21R
Gene Aliases: UNQ3121 / PRO10273, MGC10967, NILR
Product Size: 5 x 50 µg
Protein Form: Truncated, Recombinant
Protein Family: Cytokines & Receptors
Endotoxin Level: < 1 EU/µg
Protein Subtype: Interleukins, Cytokine Receptors
Molecular Weight: 26.5 kDa
Research Category: Immunology, Signal Transduction, Drug Development
Protein Tag or Fusion: His Tag
Validated Application: Assay Standard
Shipping Condition: Room Temperature

Contents & storage

Store at -20 to -70 °C

Documents

Manuals & protocols