CloneChecker™ System (for screening bacterial colonies)

Catalog number: 11666013

Invitrogen™  Related applications: DNA Extraction

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Description

CloneChecker™ System is a rapid method for screening recombinant bacterial colonies or liquid cultures of colonies for the presence of target plasmid DNA (1). The CloneChecker™ System:

• Screens bacterial colonies for plasmid DNA and insert of interest prior to purification
• Screens for inserts >200 bp in plasmids up to 15 kb by size comparison to the original vector or a DNA size marker
• Goes from plated colonies through lysis in less than 5 minutes
• Allows for supercoiled, restriction digest, or PCR analysis
• Works with single-copy plasmids starting with a 2-mm bacterial colony
• Includes sufficient reagents for processing 100 bacterial samples by supercoiled analysis (direct size comparison); and 100 samples by restriction endonuclease or PCR analysis
For Research Use Only. Not for use in diagnostic procedures.

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Specifications

High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Sample Type (General): Bacteria
Downstream Application: Cloning, PCR
Final Product: Plasmid DNA
Product Size: 100 preps
Sample Type (Specific): Bacterial Culture
Shipping Condition: Room Temperature

Contents & storage

The CloneChecker™ System includes Yellow, Red, and Green solutions and Loading buffer. Store at 15°C to 30°C.

Documents

Manuals & protocols

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