See the new Lipofectamine® 2000 Transfection Reagent protocol—now optimized for efficiency, viability and reproducibility

Lipofectamine® 2000 Transfection Reagent

Catalog number: 11668030

Invitrogen™  Related applications: Transfection

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Description

Lipofectamine® 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and suspension cell lines. Researchers use Lipofectamine® 2000 Reagent for siRNA- and shRNA-based gene knockdown experiments, as well as for gene expression studies. Lipofectamine® 2000 Transfection Reagent is our best transfection reagent available for the co-transfection of siRNA and plasmid DNA.

With Lipofectamine® 2000 Transfection Reagent, you'll get:

• Exceptional transfection efficiency in the broadest range of cell lines and the highest levels of recombinant protein expression (see table)
• Superior performance for co-transfection of siRNA and plasmid DNA
• Proven efficacy in the presence of serum—eliminates the need to change media following transfection
• Reliable performance for high-throughput applications
• The best choice for establishing stable cell lines

A high-performance transfection reagent for gene expression and gene silencing
Lipofectamine® 2000 Transfection Reagent works effectively with all common cell lines as well as with many challenging ones, and can be used in media with or without serum. For gene silencing, Lipofectamine® 2000 Transfection Reagent's high-efficiency transfections provide the high levels of gene knockdown needed to achieve convincing results. Lipofectamine® 2000 Transfection Reagent is the number one choice for co-transfection, given its effectiveness for transfecting both siRNA and plasmid DNA. Lipofectamine® 2000 Transfection Reagent is easy to use—simply mix with nucleic acid and add to cell culture.

Ideal for high-throughput work
Simplicity and speed combined with high transfection efficiency make Lipofectamine® 2000 Transfection Reagent ideal for transient protein expression or high-throughput RNAi transfections. Transfection conditions can be easily established for automated or robotic systems used in such applications.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type: Established Cell Lines, Hard-to-Transfect Cells, Stem Cells, Primary Cells
Sample Type (Specific): Plasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
Transfection Technique: Lipid-Based Transfection
Serum Compatible: Yes
Format: Vial(s)
Product Size: 0.3 mL
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice
High Throughput Compatibility: High Throughput-Compatible

Contents & storage

Each unit contains one vial of Lipofectamine® 2000 Reagent (0.3 ml). Store at 2–8°C.

Guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols

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