Platinum® Quantitative PCR SuperMix-UDG

Catalog number: 11730017

Invitrogen™  Related applications: Chromatin Biology | Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

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Description

Platinum® Quantitative PCR SuperMix-UDG is a ready-to-use mixture for high-specificity, real-time quantitative DNA amplification, containing UDG to prevent carryover contamination (1,2). The system is optimized to provide superior performance with either LUX™ Fluorogenic Primers or probe-based detection systems.

Platinum® Quantitative PCR SuperMix-UDG:

• Is highly sensitive and specific
• Allows room temperature reaction assembly (3)
• Is easy to use-just add template and fluorogenic primers or probes for detection
• Includes a vial of 50 mM MgCl2 to accommodate a variety of primer/template PCR systems
• Is compatible with most real-time instruments

Applications: Real-time quantitative amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, and fluorometric SNP (Single Nucleotide Polymorphism) detection.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Primer-Probe Detection
For Use With (Equipment): Cepheid SmartCycler, 7500 System, BioRad iCycler iQ, BioRad MyiQ, MJ Opticon, Stratagene Mx4000, Stratagene Mx3005P, Stratagene Mx3000P, MJ Chromo4, BioRad iQ5
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA
Product Size: 100 reactions
Shipping Condition: Dry Ice
Sample Type (Specific): cDNA, DNA (Genomic)

Contents & storage

Platinum® Quantitative PCR SuperMix-UDG is supplied in a 2X concentration [40 mM Tris-HCl (pH 8.4), 100 mM KCl, 6 mM MgCl2 , 400 µM dATP, 400 µM dCTP, 400 µM dGTP, 800 µM dUTP, 40 units/ml UDG, 60 units/ml Platinum® Taq DNA Polymerase, stabilizers]. A vial of 50 mM MgCl2 is also provided. Store at -20°C. Guaranteed stable for 6 months when properly stored.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

Documents

Manuals & protocols

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