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Platinum® Quantitative RT-PCR ThermoScript™ One-Step System

Catalog number: 11731023

Invitrogen™  Related applications: Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

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Description

Platinum® Quantitative RT-PCR ThermoScript™ One-Step System (1) is ideal for real-time quantitation of RNA molecules from total or poly(A)+ RNA in a single step. ThermoScript™ RT, a cloned avian reverse transcriptase, has been engineered to have reduced RNase H activity and high thermal stability (up to 70°C) to copy RNA with secondary structure and improve specificity of priming. Specificity and amplification efficiency are further enhanced through the use of Platinum® Taq DNA polymerase (3). The system can be used to quantitate the amount of specific mRNA from small numbers of cells (Figure 1).

The Platinum® Quantitative RT-PCR ThermoScript™ One-Step System:

• Is optimized to provide maximal performance with fluorescent detection chemistries employed in real-time quantitative PCR
• Provides reduced RNase H activity for greater first-strand cDNA yield
• Takes place sequentially in a single tube, under conditions optimal for both ThermoScript™ RT and Platinum® Taq DNA polymerase
• Reduces reaction-to-reaction variability for improved precision in quantitative RT-PCR
• Detects as little as 10 molecules of RNA template
• Allows precise quantification of mRNA(s) from 0.1 pg to 1 µg of total RNA
For Research Use Only. Not for use in diagnostic procedures.

Specifications

PCR Method: 1-Step qRT-PCR
Detection Method: Primer-Probe Detection
Reaction Speed: Standard
Sample Type (General): RNA
Product Size: 500 reactions
Shipping Condition: Dry Ice

Contents & storage

Platinum® Quantitative RT-PCR ThermoScript™ One-Step System contains ThermoScript™ RT/Platinum® Taq mix, and 2X ThermoScript™ reaction mix (a buffer containing 0.4 mM of each dNTP, 6 mM MgSO4 ). A vial of 50 mM MgSO4 is also provided. Store at -20°C. Guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols