Platinum® SYBR® Green qPCR SuperMix-UDG

Catalog number: 11733038

Invitrogen™  Related applications: Chromatin Biology | Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

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Description

Platinum® SYBR® Green qPCR SuperMix-UDG provides a sensitive and convenient system for real-time PCR detection with SYBR® Green I dye. The SuperMix format combines amplification and detection reagents into a ready-to-go mix to make set-up easy and ensure reproducible results. Platinum® SYBR® Green qPCR SuperMix-UDG offers:

–Platinum® Taq DNA Polymerase for sensitive amplification from as few as 10 copies of target (Figure 1)

–100% dUTP and UDG enzyme for superior control of carryover contamination, saving time by reducing the number of failed experiments
–Separate tubes of ROX reference dye and BSA for easy optimization on 96-well plates and glass capillary tube instruments (Figure 2)
–SYBR® Green I dye for simple and easy detection of real-time product
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: SYBR
For Use With (Equipment): 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, BioRad iCycler iQ, Cepheid SmartCycler, GeneAmp® 5700, StepOne™, StepOnePlus™, ViiA™ 7 System
Reaction Speed: Standard
PCR Method: qPCR
Sample Type (General): DNA
Product Size: 100 reactions
Sample Type (Specific): DNA (Genomic), cDNA
Shipping Condition: Dry Ice

Contents & storage

• Platinum® SYBR® Green qPCR SuperMix-UDG (2 × 1.25 mL)
• 50 mM Magnesium Chloride (1 mL)
• ROX Reference Dye (100 µL)
• 20X BSA (300 µL)

Sufficient reagents are provided for 100 reactions of 50 µL each. Store all components at -20°C.

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