CellsDirect™ One-Step qRT-PCR Kit

Catalog number: 11753100

Ambion™  Related applications: Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

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Description

The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.

Features of the CellsDirect™ One-Step qRT-PCR Kit include:

Efficiency—offers significant time and cost savings for increased throughput
Convenience—enzyme mix includes both SuperScript® III Reverse Transcriptase and Platinum® Taq DNA Polymerase
Sensitivity—no separate RNA isolation step increases sensitivity by minimizing sample loss
Broad detection range—detect targets from samples ranging from a single cell to 10,000 cells
Versatility—compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)

Streamline your workflow
In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect™ One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.

Ideal for your qRT-PCR application
CellsDirect™ technology is validated for gene expression studies involving limited samples. CellsDirect™ One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps (see figure). The CellsDirect™ One-Step qRT-PCR Kit may also be used to detect genomic DNA targets. CellsDirect™ One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g., TaqMan® or LUX™ fluorogenic primers).

Note: This kit includes a separate tube of ROX™ Reference Dye. An equivalent kit (11754-100) with ROX™ Reference Dye included in the 2X Reaction Mix is also available.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

PCR Method: 1-Step qRT-PCR
Detection Method: Primer-Probe Detection
Reaction Speed: Standard, Fast
For Use With (Equipment): Cepheid SmartCycler, BioRad MyiQ, MJ Opticon, Corbett RotorGene, 7500 Fast System, Stratagene Mx4000, Stratagene Mx3005P, Stratagene Mx3000P, MJ Chromo4, BioRad iQ5, 7500 System, BioRad iCycler iQ
Sample Type (General): Cells
Product Size: 100 reactions

Contents & storage

• 10 ml Resuspension Buffer
• 1 ml Lysis Enhancer
• 500 µl DNase I, Amplification Grade (1 U/µl)
• 160 µl 10X DNase I Buffer
• 400 µl 25 mM EDTA
• 100 µl SuperScript® III RT/Platinum® Taq Mix
• 2 × 1.25 ml 2X Reaction Mix
• 1 ml 50 mM MgSO4
• 2 ml DEPC-treated water
• 10 µl HeLa Total RNA (10 ng/µl)
• 100 µl ROX™ Reference Dye (store at -20°C in the dark)
The kit is shipped on dry ice and should be stored at -20°C

Documents

Manuals & protocols

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