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SuperScript™ VILO™ cDNA Synthesis Kit

Catalog number: 11754050

Invitrogen™  Related applications: Reverse Transcription

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Description

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a 2-tube format with the VILO Reaction Mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis. The kit can be used to synthesize cDNA from for a wide range of input RNA amounts.

Note: For superior first-strand cDNA synthesis performance in two-step RT-qPCR, we recommend SuperScript IV VILO Master Mix or SuperScript IV VILO Master Mix with ezDNase Enzyme. The master mix provides all cDNA synthesis reaction components in a convenient one-tube master mix format. It elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV RT and further optimized buffer. The master mix enables efficient cDNA synthesis at higher temperatures in less time and provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Reverse Transcriptase: SuperScript™ III
Ribonuclease H Activity: Reduced
Downstream Application: Real-Time Quantitative PCR (qPCR)
Reaction Format: SuperMix or Master Mix
Sample Type (General): RNA
Final Product Size(s): 10 kb or less
Optimal Reaction Temperature: 42° C
Primer Type: Random Primers
Product Line: SuperScript™, VILO™
Product Size: 50 reactions
Shipping Condition: Dry Ice

Contents & storage

• 5X VILO Reaction Mix containing random primers, dNTPs, and MgCl2
• 10X SuperScript III Enzyme Blend containing SuperScript III RT, RNaseOUT Recombinant Ribonuclease Inhibitor, and proprietary helper protein

Store in freezer (-5 to -30°C).

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