Ham's F-12 Nutrient Mix This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 11765070

Gibco™  Related applications: Mammalian Cell Culture

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Description

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free, single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Ham's F-12 is modified as follows:
With
• L-glutamine
• Phenol Red

The complete formulation is available.

Using F-12
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

cGMP manufacturing and quality system
For supply chain continuity, we manufacture F-12 at two separate facilities; one located in Grand Island, NY and the other in Scotland, UK. Both sites are compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

Specifications

Form: Liquid
Glutamine: L-Glutamine
HEPES Buffer: No HEPES
Product Size: 6 x 1,000 mL
Volume (Metric): 1000 ml
Phenol Red Indicator: Phenol Red
Sodium Pyruvate Additive: Sodium Pyruvate
Green Features: Fewer resources used, Less waste

Contents & storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Media formulations

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Manuals & protocols

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