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EXPRESS One-Step SYBR® GreenER™ Kit, universal

Catalog number: 11780200

Invitrogen™  Related applications: Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

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Description

EXPRESS One-Step SYBR® GreenER™ SuperMix kits incorporate proven Invitrogen® enzymes now specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for your primer-based qPCR experiments. These kits provide:

• Fast-activating, antibody-mediated Platinum® Taq DNA Polymerase in association with the brighter and less PCR-inhibitory SYBR® GreenER™ dye. Complete, rapid activation provides the best sensitivity and reproducibility (Figure 1).
• The One-Step SuperScript™ III Reverse Transcriptase module has a unique formulation to ensure better yield and less qPCR inhibition specifically for one-step qRT-PCR.
• Flexible format with ROX premixed or separate; designed to work with default instrument protocols on a wide range of high-throughput fast instruments (e.g., 7500 Fast, 7900 HT Fast, StepOne, Roche LightCycler® 480, Corbett Rotor-Gene™) (Figure 1)
• Peace of mind with the only commercially available one-step qRT-PCR kits containing heat-labile UDG; significantly reduces carryover contamination risk thanks to its excellent activity from room temperature to approximately 40°C. Due to the thermostability of SuperScript® III Reverse Transcriptase, the cDNA synthesis step is run at 50°C, at which temperature the heat-labile UDG is totally and irreversibly inactivated (Figure 2).

Easy to Test and Switch
EXPRESS kits work with manufacturer’s default protocols on fast instruments and come in a convenient and stable SuperMix format for ultimate convenience. Additionally, kits are offered with either premixed ROX or a separate tube of ROX to fit your instrument needs. This "Universal" kit contains a separate tube of ROX.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

PCR Method: 1-Step qRT-PCR
Detection Method: SYBR
Reaction Speed: Standard, Fast
For Use With (Equipment): StepOnePlus™, Standard Mode, Cepheid SmartCycler, 7500 System, BioRad iCycler iQ, MJ Chromo4, Stratagene Mx3000P, BioRad CFX96™, StepOnePlus™, Fast Mode, Corbett RotorGene, 7500 Fast System, Stratagene Mx4000, Stratagene Mx3005P, MJ Opticon, BioRad MyiQ
Sample Type (General): RNA
Product Size: 500 reactions

Contents & storage



EXPRESS One-Step SYBR® GreenER™ SuperMix Kits are composed of 2 modules: EXPRESS SYBR® GreenER™ RT module (containing SuperScript® III Reverse Transcriptase and RNAseOUT™) and EXPRESS SYBR® GreenER™ qPCR SuperMix with ROX in a separate tube.

Store at -20°C upon receipt. Once thawed, the EXPRESS SYBR® GreenER™ qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteed to be stable for 6 months when properly stored.

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Manuals & protocols

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