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EXPRESS qPCR Supermix, with premixed ROX

Catalog number: 1179501K

Invitrogen™  Related applications: Real Time PCR (qPCR) | Real Time PCR-Based Gene Expression Profiling

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Description

Next-generation qPCR reagents designed to complement fast-cycling instruments
Get the turnaround time you want with the data quality you need.


EXPRESS qPCR SuperMix Kits incorporate proven Invitrogen enzymes now specifically tailored to deliver superior results with probe-based qPCR assays on high-throughput, fast-cycling instruments. These kits provide:

• Fast-activating, antibody-mediated Platinum® Taq DNA Polymerase - complete, rapid activation provides the best sensitivity and reproducibility (Figure 1)
• Flexible formats with ROX premixed or separate - designed to work with default instrument protocols on a wide range of high-throughput fast instruments (e.g., AB 7500 Fast, AB 7900 HT Fast, AB StepOne, Roche LightCycler® 480, Corbett Rotor-Gene™) (Figure 2)
• Significantly reduced carryover contamination with UDG carryover protection - uracil-DNA-glycosylase (UDG)/dUTP incorporated in all kits (Figure 3)



Easy to test and switch

EXPRESS kits work with manufacturer’s default protocols on fast instruments and include a convenient and stable SuperMix format for the ultimate convenience. The kits are offered with either premixed ROX or a separate tube of ROX to fit your instrument needs.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Primer-Probe Detection
For Use With (Equipment): 7300 System, 7000 System, Eppendorf® MasterCycler® RealPlex, StepOne™, Standard Mode, StepOne™, Fast Mode, 7900HT Fast System, 7900HT System, 7700 System
Reaction Speed: Standard, Fast
PCR Method: qPCR
Sample Type (General): DNA
Product Size: 1,000 reactions
Sample Type (Specific): cDNA, DNA (Genomic)

Contents & storage



EXPRESS qPCR SuperMix kits are provided as a 2X qPCR SuperMix with ROX premixed or in a separate tube.

Store at -20°C upon receipt. Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteedstable for 6 months when properly stored.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

Documents

Manuals & protocols