This product is manufactured in our Grand Island, US facility and is intended for sale in North America, Latin America, and the Asia/Pacific region—higher freight charges will apply to orders shipped elsewhere. An equivalent product, manufactured in Paisley, Scotland, is recommended for our customers in Europe, Africa, and the Middle East. Click to view.

MEM (Temin's modification) (2X), no phenol red

Catalog number: 11935046

Gibco™  Related applications: Mammalian Cell Culture

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Description

Temin's Modified Eagle Medium (MEM), is a modified form of Minimum Essential Medium, one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco® MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With Without
• L-glutamine • HEPES
• Earle's salts • Phenol Red
• Sodium Bicarbonate

The complete formulation is available.

MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow’s MEM, MEM α, DMEM, and Temin’s Modification. Temin’s Modification is a 2x MEM with 4x the vitamins and amino acids of the original MEM. This MEM formulation contains Earle’s salts for use in a CO2 incubator. This product is made with Earle’s salts.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing
Gibco® MEM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (8.4 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

“This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in specific region, it should be used for laboratory use only.”
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Liquid
Glucose: High Glucose
Glutamine: L-Glutamine
HEPES Buffer: No HEPES
Product Size: 500 mL
Volume (Metric): 500 ml
Phenol Red Indicator: No Phenol Red
Sodium Pyruvate Additive: No Sodium Pyruvate
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Documents

Manuals & protocols

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