PureLink™ RNA Mini Kit

Description

The PureLink® RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. The PureLink® RNA Mini Kit provides:

• Rapid, non-hazardous RNA purification capabilities
• Ready-to-use RNA for many downstream applications

Rapid and convenient column purification
The PureLink® RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1,000 µg of purified RNA from a single extraction (see table). High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with no genomic DNA contamination.

Simplified, nontoxic RNA purification
The PureLink® RNA Mini Kit combines nontoxic guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can typically be completed in less than 20 minutes without the need for hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl, or alcohol precipitation.

The PureLink RNA Mini Kit is recommended to be used together with the Homogenizer (Cat. No. 12183026) designed to homogenize cell or tissue lysates via centrifugation, prior to nucleic acid purification. The Homogenizer is especially effective for clarifying particulates from plant tissues.

For plant tissues rich in polyphenolics or starch (e.g., pine needles, potato tubers), we recommend using PureLink™ Plant RNA Reagent (Cat. No. 12322012) together with the PureLink RNA Mini Kit.

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Specifications

Sample Type (General):	Bacteria, Blood, Cells, Plant Samples, Tissue, Yeast
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Downstream Application:	Microarray Analysis, Next-Generation Sequencing, Northern Blotting, Nuclease Protection Assays, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction
Elution Volume - max:	300 µl
Elution Volume - min:	30 µl
Final Product:	Total RNA
Isolation Technology:	Silica Spin Column
Number of Reactions:	10 Preps
Product Line:	PureLink™
Product Size:	10 preps
Quantity:	10 columns
Scale:	Mini
Starting Material (Amount):	5 x 10^6 to 1 x 10^8 cells, Up to 200 mg tissue
System:	PureLink™
Yield (up to):	1000 µg
Shipping Condition:	Room Temperature

Contents & storage