Notice: This product will be discontinued on September 1, 2017. For superior performance, we recommend SuperScript IV RT as a replacement.

ThermoScript™ Reverse Transcriptase

Catalog number:  12236014

Invitrogen™  Related applications: Reverse Transcription

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ThermoScript™ Reverse Transcriptase (RT) is an avian RT with reduced RNase H activity designed for RT-PCR (1). It is engineered to have high thermal stability (up to 70°C) (Figure 1), copy RNA with secondary structure, and improve priming specificity (2).

Yield—Reduced RNase H activity results in greater first-strand cDNA yields
Size of cDNA—Generation of RT-PCR products up to 12 kb
Thermostability—ThermoScript™ Reverse Transcriptase is active up to 70°C
Flexibility—Uses either total or poly(A)+ RNA

Unit Definition: One unit incorporates 1 nmol of dTTP into acid-precipitable material in 10 min at 37°C using poly(A)-oligo(dT)25 as template-primer.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.


Optimal Reaction Temperature: 65° C
Final Product Size(s): 12.3 kb or less
Reverse Transcriptase: ThermoScript™
Ribonuclease H Activity: Reduced
Concentration: 15 U&⁄µl
Enzyme Function: RNA dependent DNA Polymerase
Form: Frozen
Format: Tube(s)
Number of Reactions: 25 Reactions
Product Line: ThermoScript™
Product Size: 375 units
Quantity: 375 units
Sensitivity: Medium
Shipping Condition: Dry Ice
Template: ssRNA

Contents & storage

ThermoScript™ RT is supplied with a vial of 5X cDNA synthesis buffer [250 mM Tris acetate (pH 8.4), 375 mM potassium acetate, 40 mM magnesium acetate, and stabilizers], and a vial of 100 mM DTT. Store at -70°C. Guaranteed stable for 6 months when properly stored.


Manuals & protocols

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