pBAD-DEST49 Gateway® Destination Vector

Catalog number: 12283016

Invitrogen™  Related applications: Gateway Cloning | Protein Expression

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Description

The pBAD-DEST49 Gateway® destination vector is designed for rapid cloning with a Gateway® entry clone using lambda phage site-specific recombination and subsequent high-level, tightly regulated expression in E. coli. The pBAD-DEST49 vector features:

• The araBAD promoter for tightly regulated expression in E. coli
• HP-thioredoxin fusion for improved protein yield and solubility
• Enterokinase cleavage site for efficient cleavage of the N-terminal fusion with EKMax™
• C-terminal V5 and 6xHis tags for efficient detection and purification of fusion proteins
• R sites for efficient recombination with any attL-flanked Gateway® entry vector
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Vector Type: pBAD, pDEST
Cloning Method: Gateway®
Promoter: araBAD
Protein Tag or Fusion: His Tag (6x), Thioredoxin, V5 Epitope Tag
Antibiotic Resistance (Bacterial): Ampicillin (AmpR)
Cleavage: EK (Enterokinase) Recognition Site
Product Size: 6 µg
Inducing Agent: Arabinose
Selection Agent (Eukaryotic): None
Constitutive or Inducible System: Inducible

Contents & storage

The pBAD-DEST49 Gateway® destination vector includes 6 µg of vector. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols