Gateway® pYES-DEST52 Vector

Catalog number: 12286019

Invitrogen™  Related applications: Gateway Cloning | Protein Expression

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Description

The pYES2-DEST52 Gateway® destination vector is designed for rapid cloning with a Gateway® entry clone using lambda phage site-specific recombination and subsequent expression in Saccharomyces cerevisiae. As part of the YES™ Vector Collection, pYES2-DEST52 carries the promoter and enhancer sequences from the GAL1 gene for regulated expression in S.cerevisiae.
pYES2-DEST52 features:

• 2µ origin of replication for high-copy maintenance
gene for auxotrophic selection on economical minimal medium
• C-terminal V5 epitope and polyhistidine(6xHis) tags for efficient detection and purification
tR sites for efficient recombination with any attL-flanked Gateway® entry vector
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: GAL1
Vector Type: pYES, pDEST
Product Size: 6 µg
Cloning Method: Gateway®
Protein Tag or Fusion: His Tag (6x), V5 Epitope Tag
Antibiotic Resistance (Bacterial): Ampicillin (AmpR)

Contents & storage

The pYES2-DEST52 Gateway® destination vector is provided supercoiled and lyophilized. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.

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