AccuPrime™ GC-Rich DNA Polymerase

Catalog number: 12337016

Invitrogen™  Related applications: Methylation Analysis | PCR

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Description

AccuPrime™ GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content. The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B). Benefits of using AccuPrime™ GC-Rich DNA Polymerase:

Yield—high yields for targets up to 5 kb in length
Specificity—AccuPrime™ accessory proteins for improved PCR specificity
Sensitivity—sensitivity down to 5 ng of template DNA

Robust and specific amplifications
The AccuPrime™ buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction. The polymerase itself, from the archaebacterium Pyrolobus fumarius, has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C.

Unit definition
One unit of AccuPrime™ GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble material in 30 min at 74°C.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: AccuPrime™ GC-Rich DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 2 X
GC-Rich PCR Performance: High
Reaction Format: Separate Components
Product Overhang: Blunt
Reaction Speed: Standard
Final Product Size(s): 5 kb or less
Product Size: 200 reactions
Exonuclease Activity: 3' - 5'
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

AccuPrime™ GC-Rich DNA Polymerase (100 µL, at 2U/µL) is supplied with two 5X reaction buffers and a separate vial (1 mL) of 50 mM MgSO4 . Both buffers contain dNTPs, MgSO4 , and AccuPrime™ accessory proteins. Reaction buffer A (1 mL) is for genomic targets. Reaction buffer B (1 mL) is for use with cDNA, plasmid, or lambda targets.

Sufficient reagents are provided for 200 amplification reactions of 25 µl each (at 1 unit of enzyme per reaction). Store at -20°C.

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