This product is manufactured in our Grand Island, US facility and is intended for sale in North America, Latin America, and the Asia/Pacific region—higher freight charges will apply to orders shipped elsewhere. An equivalent product, manufactured in Paisley, Scotland, is recommended for our customers in Europe, Africa, and the Middle East. Click to view.

William's E Medium, no glutamine

Catalog number: 12551032

Gibco™  Related applications: Hepatocytes & Hepatic Models | Mammalian Cell Culture | Primary Cell Culture

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Description

William's E Medium was originally developed by Williams and Gunn as reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. This media can also be used for growing hepatocyte cells of different species, such as human HepaRG™ cells.
This William's E Medium is modified as follows:
WithWithout
• Phenol Red• L-glutamine
• HEPES

The complete formulation is available.

Gibco® William's E Medium is a modification of earlier media that has been enriched in amino acids and double the glucose. William's E Medium contains unique ingredients, including zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione and the lipid methyl linoleate.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.

Dual-Site cGMP Manufacturing
Gibco® William's E Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® William's E Medium product made in our Scotland facility (22551-022). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

William's E Medium contains no proteins or growth factors. Therefore, William's E Medium requires supplementation, commonly with 5-10% Fetal Bovine Serum (FBS). William's E Medium uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

“This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in specific region, it should be used for laboratory use only.”

Specifications

Form: Liquid
Glutamine: No Glutamine
HEPES Buffer: No HEPES
Product Size: 500 mL
Volume (Metric): 500 ml
Phenol Red Indicator: Phenol Red
Sodium Pyruvate Additive: Sodium Pyruvate
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Media formulations

Documents

Manuals & protocols

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