BaculoDirect™ C-Term Transfection Kit

      Numéro de catalogue:  12562039

      Invitrogen™  Applications liées: Gateway Cloning | Protein Expression

        Contactez-nous pour toute demande d’assistance ›

      Error lors du chargement de votre contenu!

        Numéro de catalogue
      Choisir votre mod�le de prix
      Taille unitaire
      		 Prix ({{currency}}) Disponibilité Qté
      {{product.sku}} {{product.sku}} également connu comme {{product.formattedSku}} 
      		{{product.availability.message}} —
      Ajouts
      Customize this product ›

      Votre stock sur site

      ›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} En stock)
      ›› {{supplyCenter.scName}}(En rupture de stock)
      ›› {{supplyCenter.scName}}
      This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
      Modifier le lieu sélectionné › Commander des produits non stockés
      Haut de la page

      Description

      The BaculoDirect™ Baculovirus Expression System is a powerful and versatile eukaryotic system for high-level protein expression in insect cells. The combination of Gateway™ Technology with baculovirus expression makes the BaculoDirect System the fastest and easiest method for production of recombinant baculovirus.

      How it works
      BaculoDirect™ Linear DNA (the baculovirus genome) is engineered to include attR sites for quick and efficient recombination with a Gateway entry clone. The gene of interest is recombined from the entry clone into the BaculoDirect Linear DNA using a simple, one-hour LR reaction (Figure 1). The resulting reaction mix contains the recombinant baculovirus carrying the gene of interest and is used to transfect insect cells. The need for transforming bacteria and isolating a large bacmid or co-transfecting a transfer vector and linear baculovirus DNA into insect cells is eliminated. As a result, the hands-on time is greatly reduced. Purified baculovirus can be isolated in less than one week.

      Gateway linear DNA
      BaculoDirect Linear DNA is designed for rapid cloning with a Gateway entry clone and subsequent expression in Sf9 or Sf21 insect cells. The Linear DNA features:

      • Strong polyhedrin promoter for high-level expression
      • R-sites for efficient recombination with any attL-flanked Gateway entry vector
      • TK gene for negative selection using ganciclovir
      • C-terminal V5-His tag (BaculoDirect™ C-Term Expression Kit) or N-terminal V5-His tag (BaculoDirect™ N-Term Expression Kit) for detection with anti-V5 antibody and purification with nickel-chelating resin
      • TEV protease cleavage site for removal of the V5-His tag following purification (BaculoDirect N-Term Expression Kit)
      • LacZ gene, to ensure a pure baculovirus stock is generated, which is replaced by your gene of interest after LR recombinant reaction

      Additional materials required, available separately: Gateway entry clone.

      A selection guide for choosing the most appropriate Gateway entry vector for your application can be found at: www.thermofisher.com/Gateway.

      For Research Use Only. Not for use in diagnostic procedures.

      Spécifications

      Cloning Method: Gateway®
      Product Line: BaculoDirect™, Gateway®
      Product Size: 5 transfections
      Promoter: Polyhedrin
      Protein Tag or Fusion: His Tag (6x), V5 Epitope Tag
      Vector Type: BaculoDirect Vectors

      Contenu et stockage

      The BaculoDirect™ transfection kits include five 0.3 µg vials of BaculoDirect Linear DNA, 125 µL Cellfectin transfection reagent, and ganciclovir.

      Store the BaculoDirect Linear DNA and Cellfectin at 4°C. All components are guaranteed stable for 6 months if stored properly.

      Documents

      Manuels et protocoles

      Produits recommandés

      We use cookies to ensure that we give you the best experience on our website.

      If you continue without changing your settings, we'll assume that you are happy to receive all cookies on Thermo Fisher Scientific websites. However, you can change your cookie settings at any time at the bottom of this page.

      Continue