SuperScript® III One-Step RT-PCR System with Platinum® Taq High Fidelity DNA Polymerase

Catalog number: 12574035

Invitrogen™  Related applications: Reverse Transcription

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Description

The SuperScript® III One-Step RT-PCR System with Platinum® Taq High Fidelity is designed for sensitive, high-fidelity, end-point detection and analysis of RNA molecules by RT-PCR. Using this convenient one-step formulation, you can perform both cDNA synthesis and PCR amplification in a single tube, using gene-specific primers, and target RNAs from either total RNA or mRNA.

Using the SuperScript® One-Step System
The system uses a mixture of SuperScript® III Reverse Transcriptase and Platinum® Taq DNA Polymerase High Fidelity for enhanced RT-PCR yields and fidelity, as well as the detection of longer templates. The system can detect a wide range of RNA targets from 300 bp–10 kb, and is compatible with multiplex applications. The amount of starting material can range from 1 pg to 1 µg of total RNA.

The system consists of two major components: SuperScript® III RT/ Platinum® Taq High Fidelity Enzyme Mix and 2X Reaction Mix. SuperScript® III Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme can synthesize cDNA at a temperature range of 45–60°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases. Because SuperScript® III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

PCR Method: 1-Step RT-PCR
Reverse Transcriptase: SuperScript® III
Polymerase: Platinum® Taq DNA Polymerase High Fidelity
Hot Start: Built-In Hot Start
Sample Type (General): RNA
Product Size: 100 reactions
Detection Method: Gel Electrophoresis
Shipping Condition: Dry Ice
Final Product Size(s): 10 kb or less
Primer-Probe Compatibility: Unlabeled Oligos

Contents & storage

Components:
• 100 µL SuperScript® III RT/Platinum® Taq High Fidelity Enzyme Mix
• 3 × 1 mL 2X Reaction Mix (containing 0.4 mM of each dNTP, 2.4 mM MgSO4)
• 500 µL 5 mM Magnesium Sulfate

Store all components at -30°C to -10°C.

Documents

Manuals & protocols

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