GeneBLAzer® In Vivo Detection Kit

Catalog number: 12578134

Invitrogen™  Related applications: Cellular Imaging | Protein Assays and Analysis

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Description

The GeneBLAzer™ In Vivo Detection Kit measures β-lactamase reporter activity in live mammalian cells, using the β-lactamase substrate CCF2-AM. The GeneBLAzer™ Detection System provides the following advantages:

• Suitable for use as a sensitive reporter of gene expression in living mammalian cells using fluorescence microscopy.
• Provides a ratiometric readout to minimize differences due to variability in cell number, substrate concentration, fluorescence intensity, and emission sensitivity
• Uses a non-toxic substrate, allowing continued cell culturing after quantitative analysis.
• Compatible with a wide variety of applications, including microplate-based transcriptional assays and flow cytometry.
• Provides a flexible and simple assay development platform for gene expression in mammalian cells.
For Research Use Only. Not for use in diagnostic procedures.

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Specifications

Reporter: BLA (Beta-Lactamase)
Substrate: CCF2-AM
Product Size: 375 reactions
Substrate Type: Beta-Lactamase Substrate
Detection Method: Fluorescent
Substrate Properties: Chemical Substrate

Contents & storage

In vivo detection kits contain 50 µg of CCF2-AM substrate (enough for 375 96-well reactions). Substrate should be stored at -20°C, dessicated, and protected from light. All reagents are guaranteed stable 6 months when properly stored.

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