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Invitrogen™

Platinum™ Green Hot Start PCR Master Mix (2X)

Catalog number:  13001012

 Related applications: Genotyping & Genomic Profiling | Methylation Analysis | PCR | Sanger Sequencing

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Description

Invitrogen Platinum Green Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup. It retains all the features of Platinum Taq DNA Polymerase: specificity, robustness, and reliability. Platinum Green Hot Start PCR Master Mix is supplemented with two tracking dyes for the convenience of direct gel loading of PCR products.

Product features:
• High specificity and increased yields with antibody-mediated hot-start PCR
• Convenient room-temperature reaction setup
• Direct gel loading with green PCR master mix
• Versatile formulation for broad range of amplicons

About Platinum Taq DNA Polymerase
Platinum Taq DNA Polymerase is a recombinant Taq DNA polymerase complexed with proprietary antibodies that inhibit enzyme activity at room temperature, reducing nonspecific target amplification at lower temperatures. The antibodies dissociate during the initial PCR denaturation step and DNA polymerase regains its full activity. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5'→3' exonuclease activity.

Using Platinum Green Hot Start PCR Master Mix
Convenient Platinum Green Hot Start PCR Master Mix provides a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup. The two tracking dyes in Platinum Green Hot Start PCR Master Mix enable direct gel loading of PCR products, thus eliminating tedious steps of dye addition. Platinum Green Hot Start PCR Master Mix is supplied with a separate vial of Platinum GC Enhancer formulated for specific amplification and improved yields of GC-rich targets. Use Platinum Green Hot Start PCR Master Mix in PCR applications such as genotyping, gene expression profiling, and next-generation sequencing.

Note: For superior PCR performance, we recommend next generation enzyme Platinum II Taq Hot-start DNA Polymerase. Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology. Platinum II Hot-Start Green PCR Master Mix contains Platinum II Taq Hot-Start DNA Polymerase in a ready-to-use mixture with Platinum II PCR buffer and dNTPs, thus reducing the number of pipetting steps during PCR reaction setup. It additionally contains a density reagent and two tracking dyes for direct loading of PCR products on gels.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: Platinum™ Taq DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Medium
Reaction Format: SuperMix or Master Mix
Product Overhang: 3'-A
Reaction Speed: Standard
Final Product Size(s): 5 kb or less
Product Line: Platinum™
Product Size: 50 reactions
Shipping Condition: Dry Ice

Contents & storage

Each box contains:
• 1.25 mL Platinum Green Hot Start PCR 2X Master Mix
• 1.25 mL Platinum GC Enhancer
• 1.25 mL water, nuclease-free

Store at -20°C in a non-frost-free freezer.

Documents

Manuals & protocols

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