POROS® A 20 µm Column, 4.6 x 50 mm, 0.8 mL

Catalog number: 1502224

Applied Biosystems™  Related applications: Antibody & Cell Culture Production | Antibody Purification | Chromatography | Protein Purification & Isolation

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Applied Biosystems® POROS® Prepacked Protein A, Affinity Column. POROS® Protein A is based on an immobilized recombinant Protein A functional group designed for high throughput purification of antibodies.
20 micron particle size is used for high resolution and small scale preparative to semi-preparative separation of biomolecules.

POROS® Perfusion Chromatography® Media and Pre-Packed Columns

A high performance chromatography media for analytical to process scale separations.• Higher productivity: High throughput and high dynamic capacity associated with Perfusion Chromatography®
• Chemical stability: Allows aggressive cleaning and sanitization
• Enhanced biomolecule access: Provided via large pores, ranging between 500-10000 Å
• Polystyrenedivinylbenzene particles: Yield a robust, easily packable matrix
• Develop better separations methods in a shorter time frame: The speed of Perfusion Chromatography technology reduces weeks of experimentation to only a few hours of work, so you have plenty of time to explore all the variables of your separation

Reduce time-consuming sample prep

You can replace many sample preparation steps with high speed Perfusion Chromatography technology. For instance, dialysis of large volumes of material can be replaced by high flow rate processing of the dilute sample. Elution in a small volume of new buffer accomplishes both buffer exchange and concentration.

Create novel assays for more efficient analysis

Perfusion Chromatography technology is not limited to standard modes of separation. Both enzymes and affinity ligands can be immobilized on POROS media. By combining rapid on-column protein digestions with rapid on-column immunoassays and chromatographic separations, you can create entirely new assays with unlimited potential.

High Capacity, High Resolution, High Speed

In contrast to conventional chromatography media, POROS® Perfusion Chromatography® media particles, are engineered to have two discreet classes of pores. Large "throughpores" allow convection flow to occur through the particles themselves, quickly carrying sample molecules to short "diffusive" pores inside. By reducing the distance over which diffusion needs to occur, the time required for sample molecules to interact with interior binding sites is also reduced. Diffusion is no longer limiting and flow rates can be dramatically increased - without compromising resolution or capacity. Separations can be achieved at 1,000 to 5,000 cm⁄hr compared to 50 to 360 cm⁄hr for conventional media.
For Research Use Only. Not for use in diagnostic procedures.


Format: Prepacked Column
Column Type: Analytical Column, Affinity Column
Product Size: 1 column
Immobilized Phase: Protein A
Shipping Condition: Room Temperature

Contents & storage



Manuals & protocols