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Hind III

Catalog number: 15207012

Invitrogen™  Related applications: Restriction Enzyme Cloning

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Description

5´-A↓AGCTT-3´ 3´-TTCGA↑A-5´

Invitrogen conventional restriction enzymes come with Universal Buffers L, M, H, K, or T (+BSA). Full details for the this restriction enzymes characteristics are included in the tables below.

Consider upgrading to the Invitrogen™ Anza™ restriction enzyme cloning system for superior convenience. Anza restriction enzymes utilize a single buffer and protocol—complete digestion in 15 minutes at 37°C for all types of DNA substrates. Click here for the Anza equivalent restriction enzyme.
Concentration15 U/ µL
ContentsHind III 10X Buffer M (1mL) 10X Loading Buffer (1mL)
Reaction Temperature37°C
Reaction Mixture Hind III 1 µL 10X Buffer M 2 µL Substrate DNA < 1 µg Sterile water to 20 µL
Heat inactivationEnzyme inactivated by heating at 65°C for 20 minutes.
Star ActivityUnrelated site may be cut in the presence of high enzyme concentration, or glycerol concentration >5%, and low ionic strength.
Effect of DNA MethylationNot sensitive to dam, dcm, or mammalian CpG methylation.
Unit DefinitionOne unit is the amount of enzyme required to completely digest 1 µg of DNA in 50 µL of the reaction mixture in one hour at 37°C.
10X Buffer M 100 mM Tris-HCl, pH 7.5 100 mM MgCl2 10 mM Dithiothreitol 500 mM NaCl
SourceHaemophilus influenzae Rd

Relative Activity in Universal Reaction Buffers

BufferLMHKT(+BSA)
Relative Activity (%)60*100<20200100*
* weak star activity is detected.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Enzyme: Hind III
Product Size: 5,000 units
Concentration: 15 U⁄µl
Compatible Buffer: 10X Buffer M
Methylation Sensitivity: Not dam methylation-sensitive, Not dcm methylation-sensitive, Not CpG methylation-sensitive
Optimal Reaction Temperature: 37° C
Sensitive to Heat Inactivation: Yes (Heat-Inactivatable)
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

Storage Buffer
10 mM Tris-HCl, pH 7.5
400 mM KCl
0.1 mM EDTA
1 mM DTT
0.01% BSA
50% (v⁄v) glycerol

Loading Buffer
1% SDS
50% glycerol
0.05% Bromophenol blue

Store enzyme and buffer at -20°C, and loading buffer at RT.

Documents

Manuals & protocols

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