CELLection™ Epithelial Enrich Dynabeads®

Catalog number: 16203

Invitrogen™  Related applications: Cell Isolation & Expansion

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

Use this kit to obtain up to 5-log enrichment of human epithelial tumor cells, directly from whole blood, bone marrow or PBMC. Tumor cell clumps can be enriched from bone marrow. Enriched cells are bead-free, viable and suitable for immunocytochemical staining or any other downstream application.

High enrichment of viable human tumor cells

Isolate directly from whole blood, bone marrow or PBMC
Viable cells for any cellular applications

Methodology: Add CELLection™ Dynabeads® to the sample. The beads will bind the tumor cells after a short incubation. The bead-bound tumor cells are quickly separated on a magnet and subsequently released from the beads with the supplied Release Buffer. (CELLection™ Dynabeads® contains a DNA linker that is cleaved by the DNase I in the Release Buffer.)

Note: If you want to deplete or use the isolated tumor cells for molecular applications, please use the equivalent product Dynabeads® Epithelial Enrich (Cat. No. 161-02). This product contains the same antibody, but with no release of the cells from the beads after positive isolation.

Starting Samples: Whole blood, bone marrow, buffy coat, PBMC.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Capacity: Processes ~4x10^9 cells total
Cell Type: Epithelial Cells, Tumor Cells
Isolation Method: Positive Isolation
Output Viability: >95%
Product Size: 5 mL
Reactivity: Human
Sample Type (Specific): Blood, PBMC
Starting Material (Cell #): 2x10^7 PBMCs per isolation
Target Species: Human
Shipping Condition: Room Temperature

Contents & storage

Each kit contains 5 ml CELLection™ Epithelial Enrich Dynabeads® coated with a monoclonal antibody towards the human Epithelial Cell Adhesion Molecule (EpCam). 2 vials Releasing Buffer Component 1 (DNase I) and 2 ml Relasing Buffer Component 2 are also supplied. Up to 2×109 cells can be processed with this kit. Store components at 2-8°C.

Documents

Manuals & protocols

Recommended products