This product will be discontinued on September 1, 2016. For superior performance, we recommend you switch to the Anza™ T4 PNK Kit.

T4 Polynucleotide Kinase

Catalog number: 18004028

Invitrogen™  Related applications: Nucleic Acid Labeling & Oligo Synthesis | Restriction Enzyme Cloning

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Description

T4 Polynucleotide Kinase catalyzes the transfer of the γ -phosphate of ATP to the 5´ hydroxyl terminus of DNA or RNA.
A T4 Polynucleotide Kinase Technical Bulletin is available.

Applications: 5´ end-labeling of DNA or RNA (1). Phosphorylation of synthetic linkers (1).

Source: Purified from E. coli expressing the T4 polynucleotide kinase gene in a λ vector.

Performance and Quality Testing: Ribonuclease, endodeoxyribonuclease, 3´ and 5´ exodeoxyribonuclease, and phosphatase assays;
labeling efficiency tested.

Unit Definition: One unit transfers 1 nmol of phosphate of ATP to the 5´ hydroxyl termini of micrococcal nuclease-treated DNA in
30 min. at 37°C.

Unit Reaction Conditions: 70 mM Tris-HCl (pH 7.6), 0.1 M KCl, 10 mM MgCl2 , 1 mM 2-mercaptoethanol, 0.3 mM [γ-32P]ATP,
0.38 mg/ml micrococcal nuclease-treated DNA, and enzyme in 150 µl for 30 min. at 37°C.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Enzyme: T4 Polynucleotide Kinase
Product Size: 1,000 units
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

T4 Polynucleotide Kinase is supplied with a vial of 5X forward reaction buffer [350 mM Tris-HCl (pH 7.6), 50 mM MgCl2 , 500 mM KCl, 5 mM 2-mercaptoethanol] and a vial of 5X exchange reaction buffer [250 mM imidazole-HCl (pH 6.4), 60 mM MgCl2 , 5 mM 2-mercaptoethanol, 350 µM ADP]. Store at -20°C.

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Manuals & protocols

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