DNA Polymerase I

Catalog number: 18010025

Invitrogen™  Related applications: Restriction Enzyme Cloning

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Description

DNA Polymerase I is a DNA polymerase with 5´→3´ and 3´→5´ exodeoxyribonuclease activities. DNA Polymerase I also incorporates biotinylated nucleotides.

Applications—DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5´ overhangs
Source—purified from E. coli expressing the DNA Polymerase I gene on a plasmid

Performance and quality testing

Endodeoxyribonuclease assay; efficiency of DNase I-dependent nick translation determined.

Unit definition

One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min at 37°C using template/primer.

Unit reaction conditions

50 mM potassium phosphate (pH 7.0), 6.7 mM MgCl2 , 1 mM 2-mercaptoethanol, 80 µg/mL template/primer, 32 µM dTTP, 69 nM [3H]dTTP, and enzyme in 100 µL for 30 min at 37°C.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Hot Start: No
Polymerase: DNA Polymerase I
Product Size: 1,000 units
Exonuclease Activity: 5' - 3', 3' - 5'
Shipping Condition: Wet Ice

Contents & storage

1000 units of DNA polymerase in a storage buffer of 50 mM potassium phosphate (pH 7.0), 100 mM KCl, 1 mM DTT, and 50% (v/v) glycerol. It is supplied at 5–10 units/µl.

Store at -5°C to -30°C.

Documents

Manuals & protocols

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