Taq DNA Polymerase, native

Catalog number: 18038018

Invitrogen™  Related applications: Chromatin Biology | Methylation Analysis | PCR

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3´ DNA polymerase activity and a 5´→3´ exonuclease activity (see figure). With Taq DNA Polymerase, you get:

  • Your choice of recombinant or native enzyme
  • Amplification of PCR products up to 5 kb in size
  • An enzyme that is licensed and qualified for PCR

    Applications
    Amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing

    Source
    Native enzyme is purified from Thermus aquaticus YT1.

    Unit definition
    One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
  • For Research Use Only. Not for use in diagnostic procedures.

    Specifications

    Polymerase: Taq DNA Polymerase
    Hot Start: No
    Fidelity (vs. Taq): 1 X
    GC-Rich PCR Performance: Low
    Reaction Format: Separate Components
    Product Overhang: 3'-A
    Reaction Speed: Standard
    Final Product Size(s): 5 kb or less
    Product Size: 100 units
    Exonuclease Activity: 5' - 3'
    Shipping Condition: Approved for shipment on Wet or Dry Ice

    Contents & storage

    Contains:
    • 20 µl Taq DNA Polymerase (5 U/µl)
    • 1.25 ml 10X PCR buffer (without magnesium)
    • 1 ml Magnesium Chloride (50 mM)

    Store at -20°C in a non-frost-free freezer. Stable for 6 months when stored properly.

    Documents

    Manuals & protocols

    Recommended products