BioPrime® Array CGH Genomic Labeling System

Catalog number: 18095011

Invitrogen™  Related applications: Chromatin Biology | Genotyping & Genomic Profiling | Nucleic Acid Labeling & Oligo Synthesis

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Description

The BioPrime® Plus Array CGH Genomic Labeling Systems are a high-performance labeling kits that enable reproducible labeling of genomic DNA samples for array-based Comparative Genomic Hybridization (CGH). Available in both indirect and direct labeling formats, the BioPrime® Plus Array CGH Genomic Labeling Systems provide a flexible solution to your genomic labeling needs. Using the
BioPrime® Plus Array CGH Genomic Labeling Systems, you can expect:
––High yields of fluorescently labeled genomic DNA
–signal-to-noise ratios
–detection of gene copy number variations (Figure 1)


These systems combine a highly concentrated exo-Klenow fragment of DNA polymerase I and random primers to effectively label genomic targets for sensitive genomic profiling experiments. Exo-Klenow is a mutant of the large fragment of the DNA polymerase I holoenzyme that has both 5´ - 3´ and 3´ - 5´ exonuclease activity removed. The lack of exonuclease activity makes this enzyme ideal for use in random priming protocols, enabling robust yields and efficient incorporation of Alexa Fluor® AHA fluorescent nucleotides. In the BioPrime® Plus Array CGH Genomic Labeling Systems, random octamers anneal to template DNA, providing priming sites for the exo- Klenow enzyme. Alexa Fluor® AHA modified nucleotides (direct labeling) or amino-allyl modified nucleotides (indirect labeling) are incorporated as the polymerase extends from the priming sites. The labeled samples are then purified to remove contaminants prior to denaturing and hybridization to a microarray (direct labeling systems) or coupled to the Alexa Fluor® NHS ester (indirect labeling systems) prior to hybridization (Figure 2).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: Cy™5, Cy™3, Alexa Fluor®-Labeled Nucleotides
Product Size: 30 reactions
Final Product: Probes (Labeled DNA)
Labeling Method: Direct Labeling
Labeling Target: Genomic DNA
Detection Method: Fluorescent
Includes Label or Dye: No

Contents & storage

Table 1 summarizes the components of the various BioPrime® Array CGH Labeling Systems. The BioPrime® Core Module is found in all standard BioPrime® and BioPrime® Plus Systems and Modules. It contains exo-Klenow fragment (40 U/ µl), 2.5X random primer solution (octamers), control DNA (Salmon Sperm), stop buffer, and distilled water. Store at -20°C. The BioPrime® Plus Systems and Modules contain the Alexa Fluor® modified dNTP mixes or amine modified dNTP mixes and the Alexa Fluor® NHS Esters. Store at -20°C. The standard BioPrime® and BioPrime® Plus Array CGH Genomic Labeling Systems also contain purification columns and purification buffers. Store at room temperature. All components are guaranteed for 6 months when properly stored. See Table 1 for product component summary.

Documents

Manuals & protocols

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