MAX Efficiency® DH10B™ Competent Cells

Catalog number: 18297010

Invitrogen™  Related applications: Transformation

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Description

MAX Efficiency® DH10B™ Competent Cells are highly efficient E. coli, perfect for most applications. DH10B™ E. coli are available in both electrocompetent and chemically competent formats. The high transformation efficiency makes them ideal for generating cDNA or genomic libraries. High-efficiency transformation is seen with plasmids 150 kb in size. The versatile DH10B™ genotype has the following features:

lacZ for blue/white screening of recombinant clones
• Elimination of mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries
• A1 mutation to increase plasmid yield and quantity
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Competent Cell Type: Chemically Competent
Species: E. coli
Format: Tube(s)
Transformation Efficiency: > 1x10^9
Bacterial or Yeast Strain: DH10B™
Product Size: 10 reactions
Blue-White Screening: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Reduces Recombination: Yes
Cloning Methylated DNA: Yes
Improves Plasmid Quality: Yes
Propagating ccdB Vectors: Not for ccdB vector propagation
F' Episome (to make ssDNA): Lacks F' Episome
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA): No
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Shipping Condition: Dry Ice

Contents & storage

Contains:
• MAX Efficiency® DH10B™ Competent Cells: 5 vials, 200 µl each (total of 1 ml)
• pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl
• S.O.C. medium: 2 bottles, 6 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

Documents

Manuals & protocols

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