Opti-MEM® Reduced Serum Medium, powder

Catalog number: 22600050

Gibco™  Related applications: Mammalian Cell Culture | Transfection

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Description

Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications.

This Opti-MEM® is modified as follows:

With
• L-glutamine
• Phenol Red

The complete formulation is confidential. For more information, please contact Technical Services.

Gibco® Opti-MEM® is a unique media that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® Opti-MEM® is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco® Opti-MEM® product made in our Scotland facility (22600-134). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Gibco® Opti-MEM® uses a sodium bicarbonate buffer system (2.4 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment and filtration at the time of preparation (see protocol for details).

“This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in specific region, it should be used for laboratory use only.”

Specifications

Glutamine: L-Glutamine
Phenol Red Indicator: Phenol Red
Form: Powder
Product Size: 10 x 1 L
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 30 months from date of manufacture

Documents

Manuals & protocols

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