Tfi DNA Polymerase

Catalog number: 30342024

Invitrogen™  Related applications: PCR

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Description

Tfi DNA Polymerase is a new non-Taq based, PCR enzyme derived from Thermus filiformis for use in PCR applications without any licensing fees. Like Taq, Tfi has 5'→3' exonuclease and 5'→3' polymerase activity. PCR performance is comparable to Taq DNA polymerase in yield, specificity, fidelity, and robustness (Figure 1, A and B). Tfi can be substituted for Taq DNA Polymerase in virtually any application and is ideal for genomic, cDNA, and plasmid targets up to 4 kb in length. In addition, Tfi is free of licensing restrictions that accompany Taq polymerase. Tfi can be considered for use as a component in a diagnostic application if qualified by the end user. With Tfi DNA Polymerase, you'll:



Eliminate licensing fees and restrictions associated with Taq-based enzymes

• Save money-Tfi is half the price of Taq DNA Polymerase
• Achieve yield, specificity, and fidelity comparable to Taq DNA polymerase
• No need to reoptimize - swap out Taq for Tfi
Tfi is also available in special pack sizes and for OEM customers needing PCR kit manufacturing flexibility.
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Polymerase: Tfi DNA Polymerase
Hot Start: No
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Reaction Format: Separate Components
Product Overhang: 3'-A
Reaction Speed: Standard
Final Product Size(s): 4 kb or less
Product Size: 500 reactions
Exonuclease Activity: 5' - 3'
Shipping Condition: Dry Ice

Contents & storage

Tfi DNA Polymerase is supplied with a vial of magnesium-free 5X Tfi PCR Buffer [250 mM TrisHCl (pH 8.4), 75 mM (NH4)2SO4, 25 mM KCl, proprietary stabilizers, 1 mM DTT], and a vial of 50 mM MgCl2. Store at -20°C. Guaranteed stable for 6 months when properly stored.

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