MEM α, nucleosides, GlutaMAX™ Supplement

Catalog number: 32571101

Gibco™  Related applications: Mammalian Cell Culture

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Description

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco® MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM α is modified as follows:

With
• Ribonucleosides
• Deoxyribonucleosides
• Phenol Red
• GlutaMAX™

The complete formulation is available.

Gibco® MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. Gibco® MEM α with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. This product is made with Earle’s salts.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® MEM α is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® MEM α product made in our Scotland facility (32571-093). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

“This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in specific region, it should be used for laboratory use only.”

Specifications

Glutamine: GlutaMAX™-I
Phenol Red Indicator: Phenol Red
Form: Liquid
HEPES Buffer: No HEPES
Sodium Pyruvate Additive: Sodium Pyruvate
Glucose: Low Glucose
Product Size: 10 x 500 mL
Volume (Metric): 500 ml
Serum Supplementation: Standard Serum Supplementation
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Documents

Manuals & protocols

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