McCoy's 5A (Modified) Medium, GlutaMAX™ Supplement

Catalog number: 36600021

Gibco™  Related applications: Mammalian Cell Culture

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Description

McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.

This McCoy's 5A is modified as follows:

WithWithout
• High Glucose• Sodium Pyruvate
• GlutaMAX™• HEPES
• Bacto-peptone
• Phenol Red

The complete formulation is available.

Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® McCoy's 5A is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Gibco® McCoy's 5A (modified) requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). Gibco® McCoy's 5A (modified) uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Specifications

Form: Liquid
pH Range: 6.9 - 7.1
Glutamine: GlutaMAX™-I
Osmolality: 280 - 320 mOsm/kg
Concentrated: 1 X
HEPES Buffer: No HEPES
Product Size: 500 mL
Classification: Animal Origin-Free
Volume (Metric): 500 ml
Culture Environment: CO2
Phenol Red Indicator: Phenol Red
Sodium Pyruvate Additive: No Sodium Pyruvate
Supplementation Required: Serum
Sodium Bicarbonate Buffer: Sodium Bicarbonate
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.

Documents

Manuals & protocols

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