AmpliTaq Gold® DNA Polymerase with Gold Buffer and MgCl2

Catalog number: 4311820

Applied Biosystems™  Related applications: PCR

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Description

AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase requiring thermal activation. Features of this enzyme:

• Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
• Time-released thermal activation improves sensitivity in low copy number amplifications
• Successful multiplex PCR saves time and reagents
• Includes GeneAmp® 10X PCR Gold Buffer with MgCl2

Hot-start activation
The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

Higher specificity, higher yield
AmpliTaq Gold® DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold® DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.

GeneAmp® 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold® DNA Polymerase, resulting in a highly specific, robust PCR amplification. The ionic strength and the pH of GeneAmp® 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold® DNA Polymerase.

AmpliTaq Gold® 360 DNA Polymerase for superior performance
Also available is AmpliTaq Gold® 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold® DNA Polymerase, AmpliTaq Gold® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: AmpliTaq Gold® DNA Polymerase
Hot Start: Built-In Hot Start
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Reaction Format: Separate Components
Product Overhang: 3'-A
Reaction Speed: Standard
Final Product Size(s): 5 kb or less
Product Size: 3,000 units
Exonuclease Activity: 5' - 3'
Shipping Condition: Dry Ice

Contents & storage

Contents:
• 12 tubes (50 µl at 5 U/µl) of AmpliTaq Gold® DNA Polymerase (250 units total per tube)
• 12 tubes (1.5 mL) of GeneAmp® 10X Gold Buffer (150 mM Tris-HCl, pH 8.0, 500 mM KCl)
• 12 tubes (1.5 mL) of 25 mM magnesium chloride.

Store at -15 to -30°C.

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