BigDye® Terminator v3.1 Cycle Sequencing Kit

Catalog number: 4337455

Applied Biosystems™  Related applications: Sanger Sequencing

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The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

• Improve the quality of your results for a wide range of sequencing applications
• Optimized for long read lengths
• Better dye mobility characteristics
• Improved performance reading through GT-rich regions
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
• Enhance your productivity and reduce costs

Get more uniform peak heights and improved accuracy
With BigDye® Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.
For Research Use Only. Not for use in diagnostic procedures.


For Use With (Equipment): GeneAmp® 9700, 3500xL Genetic Analyzer, Veriti® Thermal Cycler, 3730 DNA Analyzer, 3730xl DNA Analyzer, 3130 Genetic Analyzer, 310 Genetic Analyzer, 3130xl Genetic Analyzer, 3500 Genetic Analyzer
Recommended Applications: Resequencing, De Novo Sequencing - High Throughput (cDNA), Mixed-Base Detection, Comparative Sequencing (Germline Mutations - 50:50), GT-Rich or Difficult Template Sequencing, Long-Read Sequencing, GC-Rich Sequencing (>65%), AT-Rich Sequencing (>65%)
Product Size: 100 reactions
Template Compatibility: BAC DNA, Lambda DNA, Genomic DNA (Bacterial), PCR Amplicons, PCR Amplicons (50:50 Heterozygotes), Rolling Circle Amplified Products, Plasmid DNA (≤15Kb), Fosmids, Single Stranded DNA

Contents & storage

• 1 × 800µl tube of BigDye® Terminator v3.1 Ready Reaction Mix
• 1 tube M13 (-21) Primer
• 1 tube pGEM Control DNA
• 2 × 1 ml tubes of 5X Sequencing Buffer

Store kit at -15°C to -25°C. BigDye® sequencing buffer may be stored at 4°C.


Manuals & protocols

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