AmpFLSTR® Yfiler® PCR Amplification Kit

Catalog number: 4359513

Applied Biosystems™  Related applications: PCR Amplification for Human Identification

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The AmpFLSTR® Yfiler® PCR Amplification Kit permits the analysis of complex male:female DNA mixtures that often yield ambiguous or inconclusive results with autosomal STRs.

Key Features of the AmpFLSTR® Yfiler® PCR Amplification Kit:

• Analyze trace quantities of male DNA that may be present in a large background of female DNA
• Combine the inherent male specificity of Y-STRs with the robust performance of an AmpFLSTR® kit
• Amplify 17 Y-STR loci in a single PCR reaction which yields the highest discriminatory capacity of any commercially available Y-STR kit
• Search the user-friendly on-line haplotype database to easily determine the frequency of any Yfiler® kit haplotype
• Use this powerful tool for the analysis of cases that contain a suspect(s)

Combines the Inherent Male Specificity of Y-STRs with the Robust Performance of an AmpFLSTR® Kit
Mixtures of body fluids and/or tissues from a male and female are frequently encountered with sexual assault casework and other violent crimes. These mixed samples often contain trace quantities of male DNA present in a large background of female DNA. Typical samples can include vaginal swabs containing a small number of sperm cells, body swabs from female victims of oral assaults, and fingernail scrapings/clippings from female victims of violent assaults. Standard autosomal STR analysis may be unable to detect the minor male contributor in these types of samples, while the Yfiler® kit can routinely detect a minor male contributor present at less than 1:1000.

Provides the Highest Discriminatory Capacity of Any Commercially Available Y-STR Kit
The AmpFLSTR® Yfiler® PCR Amplification Kit amplifies 17 Y-STR loci in a single PCR reaction using 5-dye chemistry to provide the highest discriminatory capacity of any commercially available Y-STR kit. The Yfiler® kit can distinguish more than 98% of all unrelated males from one another, and therefore offers the discriminatory power necessary to conduct meaningful forensic DNA analysis.

A Powerful Tool for Suspect Casework
The Yfiler® kit is a powerful tool for the analysis of cases that contain a suspect(s). A significant proportion of DNA cases contain a suspect who has been identified by conventional law enforcement techniques. For these cases, the Yfiler® kit is a powerful tool that can be used to include or exclude a suspect(s) associated with an investigation.

Complete Validated Solution
Applied Biosystems® offers a complete solution for your Human Identification needs. Our system includes DNA quantification kits, a range of AmpFLSTR® kits, instrumentation, software, and consumables. The Yfiler® kit is part of the most comprehensively validated systems for STR-based Human Identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as SWGDAM and DAB.

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

“This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in specific region, it should be used for laboratory use only.”
For Forensic or Paternity Use Only.


Technique: STR (Short Tandem Repeat) Analysis
Polymerase: AmpliTaq Gold® DNA Polymerase
Label or Dye: NED™, FAM™, LIZ™, PET™, VIC®
Product Size: 100 reactions
Amplicon Size: <340 bp
Sample Volume: 25 µl⁄reaction
Reaction Format: SuperMix or Master Mix
Sample Type (Specific): DNA (Genomic), Forensic Sample
For Use With (Equipment): GeneAmp® 9700, 3130xl Genetic Analyzer, 310 Genetic Analyzer, 3100-Avant™ Genetic Analyzer, 3130 Genetic Analyzer, 3500 Genetic Analyzer, 3500xL Genetic Analyzer, 3730 DNA Analyzer, GeneAmp® 9600
Matrix and Size Standards: Matrix Standard Set DS-33, GeneScan™ 500 LIZ® Size Standard
Shipping Condition: Dry Ice

Contents & storage

• AmpFLSTR® PCR Reaction Mix
• AmpFLSTR® Yfiler® Primer Set
• AmpFLSTR® Control DNA 007
• AmpFLSTR® Control DNA 9947A
Store at 2 to 8°C.

• AmpliTaq Gold® DNA Polymerase
Store at -15 to -25°C

• AmpFLSTR® Yfiler® Allelic Ladder
Store at -15 to -25°C upon receipt, 2 to 8°C after initial use


Manuals & protocols